A Service of the Hamilton Regional Laboratory
Laboratory Test Information
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The HRLMP is committed to providing high quality laboratory results for our clients.
As one of the largest integrated laboratory medicine programs in Canada we strive to produce the right results the first time on every sample we receive.
Test Name:
Mycoplasma pneumoniae / Chlamydophila pneumoniae PCR
Alternate Name(s): Atypical pneumonia, Respiratory mycoplasma, Chlamydophila pneumoniae, Chlamydia pneumoniae, Mycoplasma pneumoniae, PCR
Requisition Form: Download requisition form for: R_708112 (2025-12-09) - LRC Microbiology Requisition - Update - Fillable.pdf
Turnaround Time: 1 day
Interpretive Comments:
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Results are reported for both Mycoplasma pneumoniae and Chlamydophila pneumoniae.

Additional Information: Mycoplasma infection may be suspected in older children with pneumonia presenting with several days of fever. In a patient with a subacute presentation with persistent cough, fever, chest soreness and radiographic evidence of pneumonia with few clinical signs, in whom other investigations (e.g. NPS for respiratory viruses, blood and sputum for routine culture) are negative, testing for Mycoplasma pneumoniae and Chlamydophila pneumoniae can be considered. When clinically indicated and in consultation with a microbiologist, CSF for mycoplasma detection can be ordered; samples are sent to the Hospital for Sick Children, Toronto, for testing.
Method of Analysis: Laboratory developed PCR for the detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae.
Test was Last Updated: 1/8/2026
Testing Laboratory: Virology-Serology
Testing Site: SJHH
Referred To: Public Health Lab